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Given the complexity of these systems, we used the Drosophila model to investigate the more conserved functions of HIF in the immune response.
In the latter case, genes encoding NRPSs whose products are involved in more conserved functions, such as the AARs, would be expected to have more stable domain architectures than those encoding proteins with niche-specific functions.
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Our finding suggests that the half-zippered structure is more ancient in SNARE evolution than any regulators that target this structure, and may have more conserved function in membrane fusion than previously thought.
Although the known roles of the mammalian GiMAPs - regulation of the survival and proliferation of lymphocyte lineages - undoubtedly reflect vertebrate-specific functions, this does not preclude older and more widely conserved functions for GiMAP proteins.
AS events detected in the brain are more highly conserved than those detected in other tissues, suggesting that they more often provide conserved functions.
However, given that foreign DNA fragments from eukaryotes frequently integrate in protist chromosomes, it should not be surprising that occasionally genes encoding proteins of a more universally conserved function such as EF2 and potentially EF-1 alpha-like [ 13] may also be co-transferred.
The search method we used was intended to identify high-confidence candidate genes for further experimental analysis that are more likely to share conserved functions within other model systems.
The 'ortholog conjecture' that at a similar degree of sequence divergence, orthologs are generally more conserved in function than paralogs—has been a prevailing paradigm, originally supported by theory rather than empirical studies.
Core conserved functions also had more temporal consistency of expression and distinct topologic features in the GRN.
This result suggested that core conserved functions do appear to have more invariance in the temporal structure of their expression program.
It is likely that targets related to fundamental systems such as reproduction, metabolic pathways or detoxification are more conserved in their functions, and therefore easier to recognize as being affected by pharmaceuticals.
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