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Both 200-nt long neighboring flanking sequences and 12-nt long TCF4 binding motifs were more conserved compared to random genomic locations, where the conservation score of the TCF4 binding motif was on average higher than neighboring flanking regions (Fig. 3B), indicating selective pressure on these motifs and pointing to functional relevance.
Among the mammals, the distances between pairs of HCEs are also more conserved compared to distances between protein coding genes.
Exonic sequences within the TERT gene were more conserved compared to the intronic sequences.
The EXPB and EXLA subfamilies are considerably more conserved compared to the EXPA and EXLB subfamilies.
However, CCE domains of plants are more conserved compared with those of animals and also contain a DAS domain.
Interestingly, we also show that G-protein subunit sequences in Pinaceae are more conserved compared to their dicot counterparts, irrespectively of subunit type.
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file 1. Normally, the entire promoter region, not only the boxes, is more conserved comparing to the rest of the leader region, which hampers distinguishing between regulated and non-regulated promoters.
3'UTRs of PK genes predominantly expressed in the nervous tissue were significantly longer and more conserved, as compared to genes expressed in the nervous tissue at low levels.
It seems that RT of CR1B elements is considerably more conserved than compared mitochondrial proteins.
The third codon has a high PBS value but with less conserved sites, while the tRNA gene cluster IQM has a lower PBS value with more conserved sites compared to other partitions.
T-test statistics on mouse/rat intronic alignments indicates, that detected elements are on average more conserved as compared to other oligos, which supports our assumption of their functional importance.
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