It showed that ZnF_C4 domain was more conservative than HOLI domain in PPAR family, supporting a more important role of PPAR zinc finger in keeping PPARs' functions [ 28].
If upon further discussion the four authors were unable to reach agreement on a risk of bias determination for a particular domain, the more conservative judgment was selected (e.g., if one reviewer made a judgment of "low risk" and the other made a judgment of "probably low risk," the "probably low risk" judgment was used).
In contrast, kinase-domain sequences are more conservative likely due to constrains imposed by the requisite interactions with other signaling partners, and were thus used in our study to simplify the interpretation of SRLK evolution.
Conservative judges get more conservative.
We report an automated algorithm that 'rescues' valuable borderline-scoring domain hits that are well-supported by domain architecture (DA, the sequential order of conserved domains in a protein query), including tandem repeats of domain hits reported at a more conservative threshold.
For cya1 even the more conservative mutation identified, already removes some of the catalytic sites in the conserved CyaA domain of the protein, and thus probably inhibit its function.
We have written a more conservative concluding sentence to this paragraph: "Thus, the apical localization of Fbxl7 requires the Ft D domain but neither the F domain nor the motif that is disrupted by the ft 61 allele".
A more conservative approach was not adopted given the lack of information known in this domain; therefore, further study is warranted.
The synteny analysis allowed us to establish a more conservative threshold for correlation of the cold tolerance score with number of Pfam domains.
We therefore defined a more conservative min_set by intersecting the max_set derived from phylogenetic iteration and the basal_set derived from the tree of domain structure.
