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The 28 indel sites were in 13 tracks with three containing three or more consecutive nucleotides.
Change in a local area is considered relevant only if two or more consecutive nucleotides show significant SHAPE changes.
In step 2 of the pipeline, the alignment of nucleotide sequences from among these two data sets was accomplished with the aid of BLASTN version 2.2.25+., The alignment resulted in two streams of output which included "matches" between viral and miR sequences, consisting of strings of six or more consecutive nucleotides, and "no-matches".
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Of the experimentally validated animal miRNA targets [ 2, 23- 28], 19 of the 24 predicted miRNA/mRNA interactions have 6 or more consecutive base pairs within the first 8 nucleotides of the miRNA; 10 of these interactions have perfect complementarity in this region.
A sequence of six or more consecutive matching nucleotides was required.
Bulges consist of one or more consecutive unpaired nucleotides on one side of an RNA duplex.
All generated sequence reads were subjected to quality control using a custom PERL script and were trimmed if they met any of the following criteria: more than three consecutive nucleotides designated as unattributable base identities (N); more than three nucleotides had a PHRED quality score less than or equal to 20; or a median PHRED quality score less than 20.
Note: as the majority of the groups with more than five consecutive nucleotides have zero L4_AND scores, inadequate sample size constrained the analysis to a maximum of five consecutive nucleotides.
If a gene showed 80% identity to another one and had no insertions/deletions of more than three consecutive nucleotides, the two genes were considered to specify the same RNA.
It is known that promoters regulated by TATA boxes are 'sharp' where transcript initiation occurs at a well defined dominant site, no more than 4 consecutive nucleotides long, while promoters regulated by CpG islands are 'broad' where multiple start sites can be detected in a broad genomic region [ 48].
The C3 Score is the total number of GSEQ-identified nucleotides that appear in runs of three or more consecutive (non-N) base calls, expressed as percentage of the length (nucleotides) of each RPM-Flu detector tile sequence.
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