The alignments of detected transcripts-to-proteins with adjusted cutoffs variably increase or decrease; this reflects more sensitive protein detection than transcript detection, but also more comprehensive transcript detection than protein detection.
De novo assembly was conducted for the individual libraries and for the four libraries pooled to maximize the coverage and to provide a more comprehensive reference transcript.
Unfortunately, we did not obtain sufficient RNA for a more comprehensive analysis of transcripts originating from Cp or Wp but, given our findings with mESCs, we hypothesize that aberrant RNA splicing and/or premature termination of transcripts was responsible for this outcome.
The reads from the three genotypes were pooled together for more comprehensive reconstruction of transcripts, and a total of 171,837 contigs were obtained from the clean reads of the pool with a mean length of 969 bp and length ranging from 201 bp to 15,537 bp (Table 3).
For a more comprehensive compilation of predicted transcripts, see Brown et al. (2014).
Among those using RefSeq input, the GENCODE set combines manual and automatic annotation and is more comprehensive by including the transcripts detected in the ENCODE project.
The use of HTTS in conjunction with microarrays allowed us to detect a more comprehensive set of soybean gene transcripts.
As an advantage, libraries provide a more comprehensive repertoire of all the transcripts and of their isoforms expressed in the tissue of origin, thereby being more explorative.
In our study, Illumina sequencing enabled a more comprehensive identification of T. pisana CNS transcripts, providing 220,602 unigenes.
In this study we have generated a much more comprehensive body of mitochondrial genomic and transcript data for two dinoflagellate species, C. cohnii and K. micrum, and these data are bolstered by a concurrent mitochondrial genomic study of the dinoflagellate A. carterae [ 20].
