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The results indicated that additional sequencing would be needed for more comprehensive transcriptome coverage.
To generate a more comprehensive transcriptome, we supplemented previous datasets with new reads corresponding to specific tissues (heads, antennae, and male reproductive tissues).
This possibility can be addressed in the future with more comprehensive transcriptome assembly and genome annotation from very large-scale RNA-seq data.
Hence, the cost- and time-effective NGS technologies will provide more comprehensive transcriptome information and facilitate the transcriptome studies, particularly in less-studied species.
To generate a more comprehensive transcriptome, here we supplement our previous thermal dataset with reads from specific tissues: heads, antennae, and male reproductive tissues.
While we assume that we obtained an almost complete gene set for root tissues, more comprehensive transcriptome data from various other tissues will be necessary to obtain a complete gene set for P. ginseng.
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Our results provide a unique and more comprehensive chicken transcriptome profile than current literature.
These large-scale ESTs will provide more comprehensive pine transcriptome information and facilitate the assembly of Pinus spp. ESTs in the future.
More comprehensive methods of transcriptome analysis could uncover RNAs that may underpin the disparity of outcome or prevalence of endometrial cancers in Blacks and Whites.
The more comprehensive human retinal transcriptome described here will likely be beneficial to studies of the genetics of retinal dystrophies and other inherited retinal disorders.
With the advent of next generation DNA sequencing technologies [ 9], such as RNA sequencing (RNA-seq), a more comprehensive and accurate transcriptome analysis has become feasible and affordable.
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