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Usually, at constant temperature, the increase of pressure increases the solvation power of the solvent; consequently, more compounds are dissolved and the global yield is increased (Kumoro and Hasan 2007; Mukhopadhyay 2000).
The two approaches should be further tested when data for more compounds are available.
However, as more compounds are profiled, the FDR for such compounds could be reduced.
The fact that more compounds are detected in their unconjugated forms in the cell lines, however, suggests that, in general, the concentrations of estrogens and EMs are higher in cells than in the circulation.
When the smaller clusters are taken into account (those with two or less compounds) the ECFP4 with Property descriptors are better than ECFP4 descriptors, and the Property descriptors are better than the GCUT descriptors, but these are both reversed when only the larger clusters (with three or more compounds) are considered.
In addition to achieving these synergistic effects, the combinations of two or more compounds are essential for the following reasons: (1) to prevent or suppress the emergence of resistant strains, (2) to decrease dose-related toxicity, as a result dosage, and (3) to attain a broad spectrum of activity [ 28].
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Three genes which were commonly regulated by two or more compounds were P53, Bcl2-associated X protein (Bax), and caspase 3 CASP3).
In phytochemical analyses, more compounds were observed in BEA, followed by EMW and CEF.
In general, more compounds were active in the antagonist-mode assays than in the agonist-mode assays, in which cytotoxicity might be playing a role.
The embryos remained in the same 4 ml egg water for the duration of the experiments, no daily refreshments of the egg water nor additions of more compound were performed.
Accordingly, molecules gathered on the map definitely characterizing much more similar compounds are more meaningful than those separated ones.
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