Moreover, in Figs. 2(b) and (c), due to the high OPD dynamic range, visibility of some details in more complex specimen is hindered thus avoiding careful recognition of internal structure and/or external filaments.
With the exception of a few very small cells (Henderson et al., 2007), more complex eukaryotic specimens must be thinned to make them transparent to the electron beam.
The aim of this investigation was to determine whether the theoretical concepts describing the basic nature of neutralization of HLA antibodies could be applied to more complex serological specimen resulting in clinically useful information.
HER2 genetic heterogeneity by ISH has been previously demonstrated on breast cancer, but it seems to be a more complex issue on GC specimens [ 39].
But the notched specimens appeared more complex failure modes, especially at 95 °C.
The stress strain behavior at increasing strain of both pre-strained and virgin specimens is more complex but it can be satisfactorily described using the concept of a strain-dependent finite extensibility parameter (introduced previously for unfilled networks).
Specimens that generated more complex patterns – including those noted in results such as the LTRS data in Notochthamalus and some TEP specimens that were found in different clades depending on the gene region analyzed – are not presented in the final analysis.
However, use of quantitative field metrics did not necessarily facilitate quality assurance of more complex biological resources, such as biological specimens, reagents, organisms, and viruses.
The situation of A. ramblae in the Iberian peninsula is more complex and difficult to interpret: some specimens had mitochondrial haplotypes clustering with those of A. ramblae from Morocco, suggesting the persistence within Iberia of some of the ancestral Moroccan haplotypes and a derived origin of most of both the Iberian A. ramblae and A. brunneus.
Current guidelines include questionnaires based on risk factors (e.g. age ≥ 45 years, BMI > 25) or the use of more complex risk scores which require invasive specimen collection [ 8- 12].
