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In many cases, existing tools only cover simple fragmentations, but not more complex rearrangement reactions [29].
Firstly, our results showed that conventional IES deletion tends to occur before more complex rearrangement events.
However, one individual contains clones from all three regions, suggesting that this genomic locus harbors a more complex rearrangement.
Finally, whereas the origin of internal deletions can easily be explained by abortive gap repair [ 51], the origin of more complex rearrangement remains elusive.
Other primers amplified parts of the N-terminus of RPK1 (or At1g72250) but did not bridge the second fusion point, which probably hints to a more complex rearrangement on this side.
The LGs that demonstrated synteny with both chromosomes could be explained in the most part by one translocation event although LG1a exhibited a more complex rearrangement that was supported by gene sequence markers (Additional file 1).
Similar(48)
Some structural methods are topology independent, meaning that they are also able to detect more complex rearrangements than circular permutation.
This may, to some extent, reflect bias in the selection of CNVs sequenced in these studies for instance, simple deletions are likely to be more amenable to amplification by PCR than the more complex rearrangements observed in some segmental duplication regions.
Furthermore, more complex rearrangements were also observed in this dataset.
The 7 mutants not producing predicted BglII restriction profiles probably resulted from more complex rearrangements.
Sequencing-based genotyping may also identify more complex rearrangements, such as inversions and multichromosomal translocations.
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