Thus it is possible, that in the context of the full length MCAK and in absence of DTT, we generate at least two MCAK conformations of neck-motor complexes that make the analysis of this MCAKE712C mutant more complex.
Dendritic tree in cul3 mutants is more complex with significantly increased branch density.
However, several lines of evidence show that the metabolic phenotype in InsP3 receptor mutants is more complex.
As the composition of the communities becomes more complex, consisting of more specialized mutants and allowing more obligatory relations to be developed between them, predictability of the growth performance of the communities from their simplest constituents vanishes [ 55].
The pattern for the Ig-like domain single cysteine mutants was more complex, with higher order complexes consistent with CMG2 trimers and tetramers (Fig 5A).
In addition the next sentence "These results suggest that the DC phenotype of foscrb Y10A embryos starts earlier and is more complex than that in yrt mutants" requires the authors to give us more details about the yrt phenotypes, which are only referenced and not adequately described.
In addition the next sentence "These results suggest that the DC phenotype of foscrbY10A embryos starts earlier and is more complex than that in yrt mutants" requires the authors to give us more details about the yrt phenotypes, which are only referenced and not adequately described.
These results show that the DC phenotype of foscrb Y10A embryos starts earlier in development and is more complex than that in yrt mutants, as the former fail in germ band retraction, lose the AS and do not progress on the zippering process.
Here we only chose TFs with simple mutant-phenotypes (Additional file 4: Table S3) to make the result more precise, ignoring complex mutant-phenotypes associated with multiple functions.
Analysis of the magnitude of the fluorescence change as a function of the concentration of phenylalanine showed that the behavior of the wild-type enzyme is more complex than that of the mutant protein, with the apparent binding of a second phenylalanine resulting in a decrease in the total fluorescence change.
