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While prokaryotes have the advantage that their simpler cell membranes and internal structure are more amenable for physical electron extraction, the more complex metabolism of eukaryotes may be more efficient and be able to support a larger diversion of redox carrier flux without undue harmful effects on the organism.
PAGE-based analyses also reveals the existence of a number of additional, previously uncharacterised pyrophosphorylated inositol reaction products, defining a more complex metabolism associated with the catalytically flexible kinase class responsible for the production of these highly energetic cell signalling molecules.
Once linkage allows the evolution of a more complex metabolism other linkage mechanism could also be explored.
Two sets of alternative SBML models (type II and type III models) were also coded to explore the effect of more complex metabolism.
This trend comes as expected for this sets because both SCt and SAp strains still retain a more complex metabolism and a set of proteins involved in cell architecture.
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The availability of efficient tools for genetic modification of LAB during the past decade permitted the application of metabolic engineering strategies at the levels of both the primary and the more complex secondary metabolism.
This is important because potential applications include more diversified communities and more complex cellular metabolisms.
These simple models showed again that biochemical pathways are complex and cultivars can vary significantly in simple primary metabolic pathways, such as amino acid metabolism, as well as more complex secondary metabolic pathways, such as phenylpropanoid metabolism.
As choline metabolism generally is more complex and variable than glucose metabolism in terms of response to therapy, one could assume that assessment of the glycolytic rate and downstream metabolites of glucose may be the most universally applicable approach for identifying relevant metabolic biomarkers.
This concept has been extended in recent years to a more complex understanding of mitochondrial metabolism and mitochondrial DNA function in aging [67].
Batch processes are simple and robust, but the only way to reach a high cell density is the fed-batch mode, which is more complex but allows the metabolism of the strain to be controlled [ 77].
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