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This case demonstrated that by using the detailed reaction SMARTS pattern, RD-Metabolizer is capable to deal with more complex metabolic reaction types and will no doubt has a broader application.
Strategies to improve the runtime performances have also been developed, which allow for more complex metabolic engineering strategies to be identified.
When combined with opportune co-emergence and involvement of polymeric catalysts, the transformation of these pathways into more complex metabolic reactions cycles with increased functionality may be an expected outcome.
Although some techniques have proved adequate for relating findings in the test tube to the situation in living organisms, study of the more complex metabolic processes, such as those involved in differentiation and development, may require the elaboration of new experimental approaches.
However, as more complex metabolic interactions are developed within polymorphic populations, new phenotypes that were not expected before are likely to emerge.
Metabolic flux analysis (MFA) of CHO cell cultures evolved from flux balancing analysis [ 12] to more complex metabolic or isotopomer dynamic models [ 13].
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These simple models showed again that biochemical pathways are complex and cultivars can vary significantly in simple primary metabolic pathways, such as amino acid metabolism, as well as more complex secondary metabolic pathways, such as phenylpropanoid metabolism.
Again, this finding indicates that a more complex biphasic metabolic reprogramming might be necessary for proper macrophage activation.
Therefore, flavonoid biosynthesis looks more like a complex metabolic grid [ 38] than a linear pathway.
Due to their large number and strong effect sizes, GIMs provide a test bed to identify and investigate more complex genetic and metabolic interactions.
Such unpredictable evolution toward diabetes seems to reflect the persistent abnormal function of the pancreatic residual tissue and, indeed, renders even more complex the postoperative metabolic evaluation of the patients.
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