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In the current study we report on the effects exerted by Q10 in membranes having a more complex lipid composition designed to mimic that of the inner mitochondrial membrane (IMM).
Nevertheless, genetic screens for in vivo fusion defects and fusion assays of isolated organelles emphasize the importance of a more complex lipid composition under physiological conditions.
This method of IR spectroscopy can now be applied to study more complex lipid and protein chemical features on the nanoscale.
The advantage of using metabolites as intermediate phenotypes is that they are more proximal to genes and biological pathways than downstream phenotypes or clinical endpoints [ 11], ensuring more statistical power to detect genetic associations compared to more complex lipid traits.
We have also shown selective interactions of the TM JM dimer with PIP2 over POPS, further emphasizing the need for more complex lipid bilayer models in simulations of membrane protein dynamics and function.
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FAs are the simplest lipids, which in turn are components of other more complex lipids.
During the lag phase of growth [23], when nutrients are abundant, lipid mobilization provides construction blocks for the synthesis of more complex lipids such as phospholipids.
The major functions of the desaturases and hydroxylases in this system are to catalyse double bond formation between carbon atoms and addition of hydroxyl groups during the biosynthesis of unsaturated fatty acids (UFAs) and the more complex lipids the sphingolipids and sterols (Huang et al., 1999; Knutzon et al., 1998; Michaelson et al., 2013; Grinstead and Gaylor, 1982; Moreno-Perez et al., 2011).
Fatty acids are found in biological systems either as free molecules or as components of more-complex lipids.
This ester bond is the principal covalent bond linking fatty acid moieties to other groups in the more-complex lipids discussed in other sections of this article.
In further studies, we investigated whether newly synthesized oleic acid could be incorporated into more complex parasite lipids such as neutral lipids or phospholipids.
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