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These results indicated that combination of different sequencing platforms resulted in longer sequence lengths and more complete transcriptome assembly.
The proteome database represents the most relevant alkaloid-producing enzymes, compared with the much deeper and more complete transcriptome library.
Using these data, we first explored the wasp genome for nonannotated transcribed features to generate a more complete transcriptome.
We chose to normalize the libraries in order to increase the likelihood that rare transcripts would be sequenced, leading to a more complete transcriptome with limited sequencing effort.
Analysis of genetic differences between the cultivars will be described elsewhere, but the data were included here to enable a more complete transcriptome assembly and analysis.
The differences we have observed between Affymetrix and MPSS transcriptomes strongly suggest that a more complete transcriptome will be obtained when both technologies are employed.
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A comparison of more complete transcriptomes may significantly reduce the latter estimate.
In contrast to their fractional occurrence in transcriptomes, the identification of such low abundant transcripts are likely to require significant resources in order to compile more complete transcriptomes.
Although the improvements and reduced cost of next-generation sequencing technologies may lead to ever more complete transcriptomes assemblies, and may have the potential to be used for identifying fast evolving transcripts with bioinformatic tools, the ISSH method will still have and interesting role to play.
Taken together, we believe the Reads-combined assemore represents a more complete consensus transcriptome assembly for inter-organ comparison.
Normalizing libraries allowed for the detection of genes also expressed at low levels and therefore a more complete reference transcriptome.
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