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We also added more complete referencing to the work of the Valencia group; see response to reviewer 3. The Guerois group has used evolutionary information to improve their machine learnt statistical potentials.
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Readers are referred to [28] for more complete references.
In insects the cationic and chloride channels have also been found but they may significantly differ from their vertebrate analogs (for more complete references, see [18], [19], [20], [21]).
Emerging whole-genome NGS data will allow the construction of a more complete reference genome.
Normalizing libraries allowed for the detection of genes also expressed at low levels and therefore a more complete reference transcriptome.
Hence, our study suggests that focusing on gene families with more complete reference databases is especially important when the choice of sequencing technology is limited to shorter reads.
Thanks to the sequencing of larger samples, such as 1000G, more complete reference panels are now being assembled, setting off a new wave of meta-analyses.
We have also made more complete reference to our earlier work in the initial section of the results to address the issues of hemichannel identity and single channel conductances.
More complete reference genomes are needed to confirm these trends and, in the case of the Canopy sample, to disentangle the influence of abundance and biomass of species and clades.
However, the recent progress of the 1000 Genomes project (http://www.1000genomes.org/) has provided the scientific community with much more complete reference panels, both in terms of the number of markers and the number of populations.
In spite of these drawbacks, PM builds of v3.5.1 will be indispensable as a more complete reference sequence for comparative mapping and syntenic studies among Cucurbits [ 34], for downstream applications such as genotyping by sequencing (GBS) [ 47], and for optical mapping that has been performed in other species to improve draft genome quality [ 48].
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