In order to more closely localize S/Ts responsible for PKA phosphorylation in-vitro, the GIRK4 C-terminus was further truncated into 4 smaller regions and subjected to PKA-cs phosphorylation in-vitro.
We have conducted research using SHRSP for over 20 years, and have found that the pathology of ONFH in this model resembles that in humans more closely, being localized in the femoral head, than in the more commonly used rabbits (Murata et al. 2007, Suzuki et al. 2008).
Importantly, it was recently shown that 11β-HSD1 is closely localized to H6PDH in the endoplasmic reticulum [10].
Several DArT markers associated with GPC were closely localized within the genome.
The lens fibers that were localized more closely to the core, and which showed intense granular staining close to their cell border by light microscopy, contained numerous electron-dense granules with an average diameter of about 500 nm.
Since Myosin 1E moves toward the barbed end of the actin filaments that would be directed toward active lamellipodial edges and concentrations of the formin, FHOD1, it is logical that Myosin 1E would localize more closely to leading edges and early adhesions (FHOD1 with β3-integrin clusters, (Kiosses et al., 2001)).
Both WT and K44H ULK3 co-localized with GFP-CHMP4B polymers at the intercellular bridge, while only ULK3 WT co-localized with GFP-CHMP4B at the Flemming body (39% vs 61% of ULK3/GFP-CHMP4B athehe midbody rings), more closely reflecting the Flemming body localization observed for endogenous ULK3 in a context without overexpression (compare to Figure 2B).
Together these data suggest that RNEW induces the formation of disc colonies, which display characteristics of localized invasion, only when cells are grown in 3-D, which more closely mimics the in vivo conditions.
The H1 C-terminal domain (CTD) localizes primarily to a single linker, while the H1 globular domain contacts the nucleosome dyad and both linkers, associating more closely with the CTD-distal linker.
