Because of a reduced number of tools for the examination of OGlcNAcylation compared to the number for phosphorylation, including in particular a limited repertoire of antibodies, the absence of convenient radiolabeling methods, and more challenging synthetic methods, the effects of OGlcNAcylation on protein structure and function are substantially less well understood.
Due to the more challenging sample preparation method, the structure of the open T4P machinery with the pilus extended was determined from only ∼300 particles at ∼45 Å resolution.
By gradually filtering out homologs with large alignment sizes from a structural dataset, the remaining data will be more and more challenging for PSC methods to classify DS and common homologs.
Achieving high-resolution typing is also becoming more challenging with traditional methods as the database of known HLA alleles increases.
1– 7 However, unsupervised methods, which do not assume any prior scene knowledge in order to help the segmentation process, are obviously more challenging than supervised methods.
In the rest of the paper, we will only consider the subject-wise evaluation method (more challenging situation).
In this set of results, we consider the more challenging scenario where the method must simultaneously recover and predict future measurements.
However, in the Colon dataset (which is recognized as a more challenging benchmark), the entropic method GR performs worse, probably due to its higher sensitivity to noise [ 34].
The application of similar knock-in/transgenic strategies for mammalian models is more challenging, but using these methods combined with the ability to express TEVp in particular tissues using cell-type specific promoters [42] and the temporal advantages of using sTEVp to control TEVp activity has the potential to provide a very powerful system to investigate protein function in vivo.
These two datasets turned out to be more challenging for all three methods, missing or merging together some similar related strains.
