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Other more challenging functional performance-based tests should be considered such as a more prolonged endurance test.
Other more challenging functional performance-based tests or self-report measures that look at higher levels of activity should be considered to discriminate between groups.
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However, in this younger group, more challenging performance-based functional tests may be required [ 4].
However, the vast majority of sequence variants map to non-coding intergenic and intronic regions, and it has been much more challenging to assess the functional nature of these variants.
This is a crucial consideration as it is generally much more challenging to find the functional impact of variants in noncoding regions.
As our results suggest, the MSI-H phenotype in 3/11 tumors from MSH6 VUS carriers is more likely to be linked to the MLH1 promoter hypermethylation than MSH6 variations found in the families, and thus, if MLH1 expression is lost in the tumor, its promoter hypermethylation should be assayed before the more challenging and time consuming functional assays.
There are also important practical issues that make functional measurements more challenging in children, including logistical difficulties, movement during the procedure, and difficulties obtaining intravenous access.
Managing minimally displaced fractures of the scaphoid waist in young patients employed in physically demanding work is even more challenging due to the issues of functional limitations and time off work.
A more challenging task is to reliably assign functional annotations to predicted coding sequences.
Future studies could utilise more challenging physical performance tasks to explore functional difficulty experienced by healthy adults with HV.
However, inferring confidence assessments for semantic networks, rather than functional networks, is more challenging, because each single type of association must be scored separately.
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