Another possible explanation for the differences observed between input cells and IPSC-derived cells in this report compared to earlier reports is that TER cells may be more differentiated, more homogenous, and more "fibroblastic" than cells generated by short-term differentiation in vitro.
After 48 hours of total culture period, expression of activation markers was more pronounced in cells generated by the 2-step differentiation and activation method.
Significantly more living cells generating ROS were in the wheat synthetics undergoing type III necrosis than in normally growing synthetics.
At visual inspection, it seems that more cells are generated and integrated into the cerebellum than in any other region of the N. furzeri brain, in line with the extensive cerebellar neurogenesis reported in other fish species (Zupanc & Horschke, 1995; Ekstrom et al., 2001; Adolf et al., 2006; Kaslin et al., 2009; Kuroyanagi et al., 2010).
Strikingly, in contrast to the WT cells that generally formed smaller colonies in subsequent passages, the miR-203 null cells generated more and bigger clones compared to WT control cells at each passage.
However, VEGF-C-stimulated Vegfr3 YFP cells generated more neurospheres.
Similarly, MeCP2 knockdown in HCT116 cells generated more aberrantly excluded exons (2 856) than included exons (1 918).
Spheres derived from CCE cells generated more aggressive and complex glandular structures in Matrigel than those from control cells.
To explore this pathway further, we next measured whether ZZ primary bronchial epithelial cells generated more EGFR ligands than controls.
Our data showed that ALDH+/CD133+ cells generated more tumourspheres than ALDH−/CD133− cells, suggesting that ALDH+/CD133+ cells possess cancer stem cell properties.
