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Ectopic expression of Radil or Prtg promotes 39±5% and 45±7% more cell migration, respectively.
The flat composite films, however, fostered more cell migration activity than the films containing topographic features.
When conditioned medium (CM) containing ERdj3 (ERdj3-CM) is added to the lower chamber of the transwell, 309±69% more cell migration is detected in AD293 cells overexpressing both Prtg and Radil.
Fibroblast proliferation and migration onto PCL/HAp films proceeded slower than on the control borosilicate glass, with the flat composite film fostering more cell migration activity than the films containing topographic features.
However, TGFβ induced more cell migration in cells transfected with BCAR3 siRNA than in cells transfected with scrambled siRNA, as determined by directly measuring the areas (9% vs. 3.5% at 36 hours; Figure 4c) or by fold change (1.9-fold vs. 1.35-fold at 36 hours; Figure 4d).
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More positively, cell migration is the driving force of embryo development and is, in adults, essential to the immune response and to the healing of wounds.Understanding cell movement, then, is important.
Such a network leads to a more efficient cell migration and proliferation and thus cicatrization.
Cell engraftment inside the scaffolds was assessed by SEM microscopy and histology, evidencing more relevant cell migration and production of extracellular matrix in C-RGD versus G-FOAM.
Overexpression of ACSL4 induced a more rapid cell migration towards the injury area in a wound healing assay.
More specifically, cell migration and proliferation both appear to be reduced during repair of CF bronchial epithelial cells compared to non-CF cells [ 99].
More complex cell migration can be assayed in three dimensions by the use of microporous membranes, first used in developmental signalling studies more than 50 years ago [ 51].
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