It effectively silenced GFP expression in 40 70% GFP-expressed HEK293 cells and it was observed that higher N/P ratio resulted in more effective silence which was likely due to better cell internalization at higher N/P ratio.
While non-viral carriers, such as GNPs, are safer and more cost-efficient as compared to viral vectors, the probability of cell internalization is lower.
Cell internalization was studied by flow cytometry and confocal microscopy.
Cell internalization of NP-FP was examined by confocal fluorescence microscopy.The antibody-conjugated NPs had a near-neutral zeta potential and remained stable in biological media for more than 1 week, which may minimizes nonspecific cell uptake.
The triggered switch of nanocarrier surface charge from positive to negative promotes cancer cell internalization.
In the presence of NaAz, 7B2-PAC can only attach to the cell membrane, without cell internalization.
The detrimental biological consequence accompanied by cell internalization was limited.
The MF66-N6L provedles proved capable cell internalization agents.
It has been reported that a stereotypical sequence of cell internalization occurs after endoderm internalization [ 9, 11].
Control studies assured that the entry of TRITC-De into DN transfected 293 cells was partially inhibited (19.7%±0.20% SD), while CA transfected cells allowed more efficient internalization (39.8%±3.39% SD).
