Transcripts including exon IB predominantly contained exon IIL and were more broadly expressed, with relatively higher expression levels in the heart, thymus, and spleen.
Of the remaining lines, 168 (69.1%) showed expression in <20 of the tissues, suggesting a relatively specific expression pattern, whereas 58 (23.9%) were more broadly expressed (≥20 tissues with detectable lacZ expression).
This expression pattern of Gomafu correlates with Celf3 expression, although Celf3 is more broadly expressed in almost all of the postmitotic neurons in the brain (Shinichi Nakagawa, unpublished observation).
Btg2 was found in subsets of ONBL cells at E12.5 (Figure 14E) and E16.5 (Figure 14F), but its expression appeared to become more broadly expressed at P0 by section ISH (Figure 12G).
SV2B mRNA expression shows developmental variation, being more broadly expressed in neonatal brain than adult brain consistent with it playing a role in synapse development [8].
Thus, even though A1 and E2 belong to a class of more broadly expressed subunits they appear to require Foxi1 for expression of their mRNA in these cell types.
We think that there are few genes that fit this third category; known activators at this stage tend to be more broadly expressed, and loss of function hairy mutants show widened expression of target genes (stripes expand), consistent with broadly expressed activators.
This work highlights the importance of interactions between lineage-specific cardiac transcription factors (e.g., NKX2-5) and more broadly expressed co-factors (e.g., ETS) in the regulation of gene expression.
In the diencephalic ventricle (Fig. 1j) and the dorsal habenula (Fig. 1k) all cells were co-labeled with Elavl3 (magenta) and HA (cyan) while Elavl3/4 (green) was more broadly expressed, suggesting that HA-Uprt-mCherry largely recapitulated endogenous Elavl3 protein expression and not the broader Elav3/4 pattern.
The review finds that there are two related, non-conflicting, schools of urban metabolism: one following Odum describes metabolism in terms of energy equivalents; while the second more broadly expresses a city's flows of water, materials and nutrients in terms of mass fluxes.
In this chimera, archaebacterium-derived genes, despite being fewer in number, occupy more important positions, being more likely to be lethal upon inactivation and more highly and broadly expressed and encoding proteins that occupy more central positions in the PIN.
