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If this change increased the MCS in the updated motif, we repeated this process for one more base position.
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Sequence analysis also showed that cyt b contains 21.3% more base positions (relative to the size of the gene, largest variant) that are variable than does COI.
All of the healthy control subjects showed variants at >0.2% heteroplasmy in both the blood and skeletal muscle DNA samples at one or more base positions.
This includes 81 sequences where the primers are as expected, but positioned one or more base pair positions off the 5' end of the sequence.
The nonresponders had more mixed base positions than relapsers both pre- and post-therapy (p = 0.004 and p = 0.007 respectively) (Figure 8A).
This is a valid concern when working with libraries that contain twelve or more random base positions which are characterized by a complexity (∼10) that is near the number of beads used here (∼10).
Primers were regarded as being highly conserved if they had no more than one base position mismatch.
(3) Because mutants with wild-type bases at both −57 and −52 had greatly reduced activity, we conclude that one or more bases at positions −56 to −53 are important for promoter activity.
Recently, several studies employed computational methods to analyze the published functional siRNA datasets whose sizes are relatively large and revealed more base preferences at particular positions [ 17- 20].
A described in Materials and Methods, specific mutations at the same base position occurring more than once per animal was considered as one single mutational event.
The average quality score was more than 30 at each base position in all the three data sets except for last seven bases at the 3′ end in PE data set.
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