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Moreover, two more balanced datasets were also built as subsets of the main one.
More complete sequence data, especially from M and L segments, allowing construction of more balanced datasets for all three segments, would be very helpful to better understand the complexity of DOBV evolution.
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In addition we attempted to derive a more balanced dataset in some of the MLP modelling by including in the very small number of non-sensitisers, those that were classified as weak sensitisers from the original dataset.
In order to provide more balanced dataset we also looked at binary classification models using the same data but incorporating the "weak sensitisers" in with the "NC" compounds in the "non-sensitiser" category.
We would have preferred a more balanced dataset for better data modelling.
This appeared to be more effective when high and low vulnerability were delineated using a lower threshold, resulting in a more balanced dataset [ 32].
In order to investigate the adequacy of the methods proposed here with a more balanced dataset, the same simulation parameters as described in the previous section were used to simulate five generations of an artificial population with 25 000 records.
In order to obtain a more balanced "case control" dataset for stage 1, we also included an additional 322 "control" subjects without CAD that were selected from GeneBank.
To make the latter dataset more balanced, 30 grade III tumors were randomly selected to compare with the 15 grade I tumors.
Therefore, the use of balanced datasets is more preferable.
Researchers are advised to undertake mediation analyses in more than one balanced dataset [ 24].
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