Sentence examples for more ambiguous bases from inspiring English sources

Additional quality filters removed any tag that has one or more ambiguous bases (Ns), and any tag that is less than 50 nucleotides in length [14].

First, we removed any reads with two or more ambiguous bases (Ns) or mean Phred quality score less than 20.

Promoters less than 1000 bases or containing two or more ambiguous bases (N) were removed from the analyses.

We used the NGS QC toolkit (57) to discard reads that contained five or more ambiguous bases as well as those that contained eight or more homopolymer stretches.

The paired-end reads for each of the 96 samples were pre-processed in order to discard any pair in which at least one read contains one or more ambiguous bases.

NCBI reference sequences containing one or more ambiguous bases ('N') were removed from further analysis, such that 4,363 mRNA and 294,896 EST sequence files were available for the assembly.

These raw reads were filtered by quality and for adapter contamination using an in-house pipeline at BGI, targeting reads containing adapter sequences, those with more than 5%% ambiguous bases, or those with more than 50%% of bases with a Phred quality score below 10.

Quality trimming of raw GSFLX and Illumina reads was performed using custom Perl scripts according to the following prescription: a) reads were trimmed back if they contained more than three consecutive ambiguous bases, or more than three consecutive bases with a PHRED score <20; b) reads were discarded if the median PHRED score was <20; and c) reads were discarded if the length was <50 bp.

Using ARB, I removed any sequence that affiliated with mitochondria or chloroplasts or were flagged as being of poor alignments, having more than five ambiguous bases, or appearing chimeric.

Selected representative sequences contain no more than five ambiguous bases in the V1-V3 region and span the entire V1-V3 whichw, whash han averageage length of ~485 bases.

Briefly, sequences that were less than 200 bp or greater than 1000 bp in length contained incorrect primer sequences, or contained more than 2 ambiguous bases were discarded.