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We searched gene loci with two or more alternative splicing isoforms supported by FL-cDNAs to determine whether alternative splicing mechanisms affect gene expression during Pi stress.
More alternative splicing occurs in human cells than in those of lower animals like flies and worms, Dr. Crollius said.
More alternative splicing goes on in human cells than in those of lower animals like flies and worms, Dr. Crollius said.
By analyzing these genes, we showed that genes with alternative promoters tended to demonstrate alternative splicing compare to genes with single promoter, and, genes with more alternative promoters tend to have more alternative splicing variants.
The system allows users to compute isoform signatures for one or more alternative splicing isoforms at the same time, offering a variety of different methods to describe the isoform structure, and provides its results as a web page or as a downloadable delimited file.
In particular, disease genes have longer coding sequences, more exons and more alternative splicing [ 19].
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More alternative spliced genes would be discovered if these alternative splicing models were considered.
231 genes were identified with two or more alternative splice variants, mostly due to intron retention.
Moreover, 231 genes were identified with two or more alternative splice variants.
Second, aided by improved sequencing technology and increasing EST and cDNA experimental data, many more alternative splice isoforms had been identified [ 17, 18].
In contrast to mammalian organisms, we found that duplicated genes in rice and Arabidopsis tend to have more alternative splice forms.
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