We also found that RNA-seq is more sensitive in reproducibly detecting alterations in gene expression, detecting more genes altered at lower quantitative levels (Additional file 1: Table S1; 5 > 2 fold).
Conversely, females showed more genes altered at higher doses, and had only 43 transcripts altered at all four doses (Fig. 2e).
The better separation of non-oncogenic and cancer cell lines at isoform-level (97.5% cluster purity) compared with gene - 75% cluster purity) implies that gene-expression profiles in cancer cells are more specifically altered at isoform-level for numerous genes, which could not be detected using gene level analysis.
The optimal age to intervene and reduce excess weight has yet to be established, but there is currently considerable interest in targeting younger children [ 2], based on the premise that behavioural patterns that may be encouraging weight gain might be more easily altered at this age rather than becoming firmly entrenched.
This observation and the fact that stimuli were not panic-related suggests that processing of emotional stimuli may be altered at a more general level.
In the epididymal WAT depot, 2037 genes were altered at one or more time points in response to HFD intake over 24 weeks (Table 1), of which 1610 genes were up-regulated and 445 genes were down-regulated.
In mesenteric WAT depot, 1784 genes were altered at one or more time point in response to HFD intake over 24 weeks, of which 1134 genes were up-regulated and 892 genes were down-regulated (Table 1).
In critically ill patients more than one variable may be altered at the same time, as in sepsis where the impairment of heart contractility and the decrease of intravascular volume due to capillary leakage may be associated with the decrease of the primary artery vessel tone.
Blood pressure is unlikely to be altered at earlier time points and is technically more difficult to measure.
However, simultaneously, these mechanisms may be altered at any point, favoring plasticity and producing a more effective immune response.
