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Libraries of bacterial chromosomal fragments were screened using two separate systems that monitor: (i) elevated fluorescence conferred by enhanced expression of GPCR GFP fusions and (ii) increased binding of fluorescent ligand in cells producing more active receptor.
Shorter CAG repeat polymorphisms (more active receptor) in AR are associated with higher serum androgen levels among premenopausal women [ 30].
MCF7 cells express a wild-type AR, albeit with a shortened CAG repeat [ 26] that is often indicative of a more active receptor [ 27].
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The ErbB3 cell-surface receptor, historically overlooked due to its perceived lack of catalytic activity, has been thought to be inert, functioning only as a scaffolding partner for other more active receptors.
The present investigation represents a first study dealing with the interaction of BPA with the biologically more active somatostatin receptor subtype (sst(2)) in the rat limbic circuit.
All IgGs exhibiting receptor colocalization triggered changes in receptor conformation (determined with fluorescent sensors) not stringently correlated to cAMP stimulation, suggesting the induction of more or less active receptor conformations.
On the other hand, steroid receptor positive and mostly luminal tumors of Cluster A or B, with more active growth factor receptor-RAS-MAPK signaling, are more likely to retain the variable exons of the CD44 precursor transcript.
The Nrf2/Keap1 pathway is more active in steroid receptor-positive disease and this subsequently causes induction of antioxidant defense that is not observed in TNBC.
Yet to be fully appreciated, the entity that stores, stabilizes, and presents growth factors in more active configurations to their receptors is not heparin, but rather the heparan sulfate chains associated with proteoglycans on cell surfaces and the ECM (Decarlo et al. 2012; Casu et al. 2010).
Conversely, when we get too little sleep, this receptor becomes more active and makes T-cells less sticky.
Cyclodextrin-curcumin complex was also more active in inducing the death receptors and apoptosis of leukemic cells as well as other cancer cell lines.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com