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In particular, there was a significant decrease in catalase and SOD activities, the more active enzymes in P. richtersi.
Thus, the deletion of pancreatic loop in venom PLA2 enzymes plays an important role in the evolution of catalytically more active enzymes.
Acetyltransferase from F. sporotrichioides, which was probably selected in the past because of intellectual property issues rather than for scientific reasons, is likely to be replaced by more active enzymes.
The fact that this relative increase in activity is smaller than that seen for the combination of GH9 and GH8 modules in a single construct to make the multifunctional peptide may reflect the diminishing returns encountered when more active enzymes, having progressed further through the substrate, subsequently encounter steadily more recalcitrant material.
Cooking your own garbanzos and using fresh fruits, etc. would allow more active enzymes while lowering the salt.
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This is more than an order of magnitude greater than the values measured here by ITC for the human enzyme, but, as described below, it has been shown recently that a much more active enzyme that is not putrescine-activated is formed by a heterodimer between T. cruzi AdoMetDC and a structurally homologous regulatory protein (51).
A comparison of activation energies for both systems indicates that the immobilized enzyme system requires more active enzyme compared to its free counterpart for same GOS yield.
YjgB appears to be the more active enzyme; however, its production seems to be only induced when the gene coding for YahK is knocked out.
It is interesting to note that the combination of the mutations K316N and D500G resulted in a more active enzyme relative to single mutants.
Dcp2 catalysis is promoted by the closing of the bi-lobed Dcp2 structure to create a more active enzyme and more stable substrate binding (Deshmukh et al. 2008; She et al. 2008; Floor et al. 2010).
This mutation is in the leader sequence that controls transport of the enzyme into the mitochondrial matrix, and Ala16 is thought to facilitate enzyme transport and lead to a more active enzyme.
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