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Similarly, at 4 months of age, more active caspase 9-positive cells (9.2±1.0 per section) were present in the Psen cDKO neocortex, relative to the control (4.2±0.3 per sections; p<0.0003; Fig. 2C).
A higher percentage of CD4+CD25+high T cells in recent-onset T1D subjects produced more active caspase 3 than the same T-cell subtype in control subjects, confirming the ongoing apoptosis detected by YOPRO1/7AAD.
At 2 months of age, more active caspase 9-positive cells (7.1±0.3 per 20 µm sagittal section) were found in the Psen cDKO neocortex, compared to the control (4.1±0.1 per section; p<0.002; Fig. 2C).
Tadpoles exposed to 35 mg/L atrazine had significantly more active caspase 3, and thus apoptosis, in the kidneys than the paired DMSO control after 24 hr (Wald χ = 5.78, p = 0.016) and 48 hr of exposure (Wald χ = 14.73, p = 0.0001;.
The more active caspase 3/7 is present in the cells, the more fluorescent dye is cleaved from the non-fluorescent substrate (Z-DEVD-R110) and differences in emission of varying culture conditions can be measured at 521 nm in a fluorescence plate reader (Tecan Group AG).
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Double stainings showed more active caspase-3 than TUNEL-positive BEC after 30 min of OGD.
There was more active CASPASE-8 associated with RIP1 in the NEMO-null/IκBαSR cells compared to the NEMO-WT/IκBαSR cells.
This calpain generated caspase-7's 17.2-kD fragment is ~18 fold more active than the 20-kD caspase-3 generated fragment of caspase-7 [ 116].
In turn, active caspase 9 activates the downstream caspase cascade that involves caspase 3 and other effector enzymes.
In contrast, Hela cells grown for 7 days under the same culture conditions demonstrated that many more cells (7.5%) were positive for active caspase 3 (figure 2a) and were also positive at day 4 (results not shown).
For each tumor, more than 250 cells were counted for active caspase 3 positivity (brown cellular cytoplasm) and the percentage calculated.
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