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Less robust cultures with 80 150 osteoclasts per well were more accurately quantitated by counting individual osteoclasts (Fig. 2).
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To more accurately quantitate gene expression, we isolated spontaneous tumors from the C3(1) TAg and MMTV-Neu models and measured mRNA levels by quantitative reverse transcription and polymerase chain reaction (qRT-PCR; Figure 1c, left panel).
In addition, the efficiency of coreceptor use can be accurately quantitated with flow cytometric analysis.
This raises the question if circulatory, fragmented DNA can be accurately quantitated by real-time PCR when high-quality genomic DNA is used as a quantitation reference.
RNA samples were accurately quantitated using a Nanodrop Technologies ND-1000 spectrophotometer (Wilmington, DE).
Here, we have accurately quantitated differentiated parasites in chronic infections, enabling the key parameters governing trypanosome infection dynamics to be dissected objectively.
In addition, the late binding tertiary protein S21 could not be accurately quantitated, likely due to its extremely low levels in the purified intermediates.
However, enzyme histochemistry is difficult to accurately quantitate and is usually regarded as a semi-quantitative technique.
Real-time quantitative RT PCR fulfils this requirement and has recently been used to accurately quantitate transcription levels of a variety of genes (Heid et al, 1996).
We anticipate sequential integration of NP-guided "Detection units" that can rapidly and accurately quantitate the transient level of metabolites and secretomes released by the cells grown in an organ chip (Fig. 4b, c).
It is technically very complex to accurately quantitate the proportion of polyploid cells within the epidermis.
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