Sentence examples for more accurate normalization from inspiring English sources
Such adjustment results in better fit of the normalization curve and consequently in more accurate normalization.
Our results showed that the elimination of potential DEGs is essential for more accurate normalization of RNA-seq data.
For more accurate normalization, use of at least two of the top ranked reference genes followed by geometric averaging is recommended for determining a normalization factor.
Based on a recommended cut-off V value of 0.15; ubiquitin was selected as a single standard reference gene for these experiments as the use of additional reference genes did not contribute to a more accurate normalization factor.
In addition to 3 references genes suitable for transcript normalization in the whole developmental series, we recommended other combinations of internal controls that provide a more accurate normalization in studies focused on less heterogeneous sample panels.
Although the advantage of using both reference genes for normalizing is not clearly evident than using the most stable gene TBP alone in our study, a more accurate normalization was found by other investigators when more than one reference gene was used [ 4, 33, 35].
This non-specific staining is unlikely to have a constant ratio to neuronal staining in a complex tissue such as the cortex, and therefore, functional normalization may provide a more accurate normalized measure compared to ΔF/F.
The coregistered MR images were processed using the DARTEL (Diffeomorphic Anatomical Registration Through Exponentiated Lie algebra) approach [32] to enable a more accurate spatial normalization.
Therefore, in most cases, the use of three reference genes results in much more accurate and reliable normalization than only one gene and is thus considered to be a valid normalization strategy.
We attribute this to more accurate quantitation and normalization of transcript abundances, owing to the use of spiked transcript controls, as well as the use of larger arrays.
In summary, the new cotton reference genes will enable more accurate and reliable normalization of qPCR results for gene expression studies in this important crop plant.
