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The 454/Roche contains a relatively high indel count, but has much more accurate base calls.
The resulting BAM files contain not only more accurate base quality scores but also more widely dispersed ones.
The 454 approach provides more accurate base calling and deeper sequencing coverage than is possible with conventional Sanger sequencing while dramatically decreasing labor [ 1- 3].
However, the more accurate base calling and deeper sequencing coverage of the 454 approach means that transcribed genes of non-model organisms can be characterized without a pre-existing sequence reference.
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In addition, Shendure et al. [17] state that a simple comparison between 'per base' costs may be misleading, because statistically more accurate bases may be worth more than less accurate bases.
Moreover, Obama's view of the threat from terrorism is more accurate, based on facts and rational analysis.
Conversely, more accurate base-callers reduce the coverage required to reach a given accuracy and therefore directly decrease the sequencing costs.
These limitations can potentially be overcome in the future by target enrichment methods such as capture probes to increase coverage, improvements in nanopore sequencing technology, or more accurate base-calling and alignment algorithms for nanopore data [ 43, 44].
In acute settings short-term prediction may be more accurate based on global clinical judgment [ 9- 11], or rating scales specifically developed to assess short-term prediction of aggressive incidents [ 12].
RNA-Seq is a promising replacement for microarrays as initial studies have shown that RNA-Seq expression estimates are highly reproducible (Marioni et al., 2008) and often more accurate, based on quantitative PCR (qPCR) and spike-in experiments (Mortazavi et al., 2008; Nagalakshmi et al., 2008).
On the other hand, the diversity usually conflicts with the accuracy of base learners; that is, the more accurate the base learners are, the lower the diversity among them is.
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