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To further illustrate the usefulness of RNA-seq for determining transcript abundance, we searched for another repeated genes among the more abundant transcripts listed in Table 2.
GSU2075, which is predicted to encode a subtilisin enzyme (a proteolytic enzyme, serine endopeptidase) and which is in an operon with omcZ [38] also had more abundant transcripts in the current-producing biofilm.
It is apparent with the One-Direct system however that a greater number of more abundant transcripts are not detected as RNA input is further progressively decreased (10 pg), as might be expected at this low (essentially single-cell) level of input.
The identity of the more abundant transcripts (Table 1).
Slowly evolving paralogs also produce more abundant transcripts.
A detection threshold would bias analyses toward detecting the proteins encoded by more abundant transcripts.
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Ancient MIRNA genes had more abundant transcript signals on both sense and antisense strands, especially on the region of 200 n.t.
A lower delta Ct value indicates more abundant transcript.
A lower delta Ct value indicates a more abundant transcript and vice versa.
The chromatin association signal for Lethe is also significantly greater than that of GAPDH, a far more abundant transcript with presumably more copies of primary transcripts.
As 'alternative transcript 1' was found to be the more abundant transcript, we used it as the template for our mutation screening experiments.
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