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Paternal and maternal deletion mice and wild-type littermates (aged 3 4 months) were implanted for chronic EEG monitoring.
Nine wild type (WT) and 13 transgenic (TG) mice (age 3 6 months) were implanted under isofluorane anesthesia with telemetry transmitters (ETA-F20, 3.9 g weight, Data Science International, St . Paul MN) capable of acquiring and sending electroencephalograph (EEG), temperature, and movement data.
Adult Nova2 and wild-type mice (aged 6 9 months) were implanted for chronic EEG recordings.
Two SCID mice (age 6 months) were implanted subcutaneously on the lower, right flank with 2.5 × 10 FaDu cells.
Adult Lhx6 LacZ/+ and Lhx6 LacZ/− littermate mice of either sex (aged 3 8 months) were implanted with electrodes for chronic recordings.
For chronic in vivo imaging, APPPS1/GFP transgenic mice at the age of 2.5 months were implanted with a cranial window as described above, without application of the antibody and the dura mater left intact.
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Mice (n = 4, 5 months old) were implanted with electrodes in the CA1 subfield of the hippocampal formation, and in two distant neocortical regions (frontal and occipital cortex) under stereotaxic guidance.
Five male Long-Evans rats (4 6 months old) were implanted under anesthesia (induction: ketamine (50 mg/kg) and xylazine (6 mg/kg); maintenance: isoflurane 0.5 3%,) with 2 arrays of independently movable recording tetrodes (for detailed methods, see Jones and Wilson, 2005).
Despite a median age of diagnosis of 6.1 (IQR: 3.3, 9.1) months, these children were implanted at a median age of 20.6 (IQR: 14.0, 29.4) months.
Rats of 7 months of age were implanted with either a single four-shank 54-channel silicon probe targeting the left mPFC or a single four-shank 64-channel silicon probe targeting the left VS.
The results of stiffness test of single staple state in Table 4 showed that 4 months after the SMA staples were implanted into the vertebrae bodies, the stiffness of the spine and the stability were increased.
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