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For measurement of muscle weights, pectoralis, triceps, quadriceps and gastrocnemius muscles from both sides of mice aged from 4 weeks to 8 months were dissected, and the average weight was used for each muscle.
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The pancreas of three to five Tg nkx6.1 eGFP) adult fish (6 9 months old) were dissected and collected in HBSS with calcium.
Seven proximal femurs from four young (5 months) porcine hips were dissected and carefully cleaned of soft tissue, muscle and capsular attachments.
The proximal femur from nine young (5 months) porcine hips were dissected and cleaned of soft tissue, muscle and capsular attachments.
After two months, all mice were dissected.
Testes from Bscl2+/+ and Bscl2−/− males (4 months old, N=3) were dissected and the tunica albuginea was removed to expose individual seminiferous tubules in 1 × PBS.
18 months old mice brains were dissected to use one hemisphere for RNA, the other hemisphere for protein extraction.
Mice were euthanized at 4 months of age, and hippocampi were dissected and frozen in liquid nitrogen immediately.
For NMJ structural analysis, Mbnl1+/+, Mbnl1−/− and Mbnl1−/−; Mbnl2+/− (4 5 months of age) TA muscles were dissected and fixed in 2% paraformaldehyde at 4°C overnight and then the myofibers were teased off into 5 10 thinner muscle bundles.
For western blot of Ddit4 tissues were dissected from six months old, male C57Bl/6 J mice that were fasted overnight or continuously kept on normal chow diet.
For quantification of cardiac hypertrophy, adult hearts were dissected at 6 months of age, fixed in 4% PFA then sectioned and stained with H&E.
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CEO of Professional Science Editing for Scientists @ prosciediting.com