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Death at 3 months was ascertained by scripted telephone follow-up, state death certificates and electronic medical records with prior patient authorization.
Use of dietary supplements during the previous 12 months was ascertained through two questions.
HIV sero-status in adults and children > 12 months was ascertained via antibody testing from finger-prick blood samples using a serial algorithm with Determine® as first test and Unigold® as a confirmatory test [ 36, 37].
Dietary habits during the 6 months preceding enrolment were ascertained through a validated food-frequency questionnaire that also covered intake quantity of about 80 food items and beverages (Wolk et al, 1998).
At each follow-up (i.e. at 3, 12, and 24 months) the respective severity ratings were ascertained.
Incident falls were ascertained every 4 months by postcard.
Events were ascertained every 4 months.
All outcomes were ascertained through 30 months.
Changes in treatment regimen were ascertained within three months of the index test.
The vast majority of cases were ascertained within 1 3 months of diagnosis; physician consent to contact patients was generally obtained within 1 month, and approximately 70% of questionnaires were returned within 2 months of mailing.
Deaths were captured at the biannual follow-up assessments (at 6, 12, 18 and 24 months) and exact dates of death were ascertained from the treating primary care doctors.
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