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Six, male, retired breeder, Sprague-Dawley rats aged 8 months were anesthetized via isoflurane inhalation.
Briefly, mice aged 3 5 months were anesthetized with a mixture of ketamine (100 mg/kg) and xylazine (10 mg/kg) by intraperitoneal administration.
WT and KO adult mice (6 months) were anesthetized with pentobarbital (Ceva Santé Animale; 0.15 ml/ 10g body weight) and perfuzed intracardially with 9% (w/v) saline buffer, and brains were placed in Golgi-Cox solution [56] at room temperature in the dark for 10 days.
Male mice ages 2 4 months were anesthetized with ketamine and xylazine by i.p. injection and positioned in a stereotaxic frame.
Mice at the age of 3-4 months were anesthetized with ketamine (100 mg/kg) and xylazine (10 mg/kg) and transferred to a heated platform.
Mice at the age of three months were anesthetized by intraperitoneal injection of a mixture of 40 mg/Kg of ketamine and 10 mg/kg of xylazine and ovariectomized using a dorsolateral approach.
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Athymic nude Foxn1nu mice (2 months old) were anesthetized under 1% to 2% isoflurane and placed on a heating pad maintained at 37°C.
Zebrafish from AB strain at various stages (1.5, 3, 6, 18 and 24 months old) were anesthetized with 0.05% benzocaine.
PDE3B KO and WT mice (3 5 months old) were anesthetized with an intraperitoneal injection of midazolam (Dormicum, Hoffman- La Roche, Basel, Switzerland) and a mixture of fluanison/fentanyl (Hypnorm, Janssen, Beerse, Belgium).
Adult mice (∼2 months old) were anesthetized by i.p. injection of 2,2,2 tribromethanol (15 mg/kg), eyes were enucleated and immersion fixed in paraformaldehyde 4% for 15 min, washed in 0.1 M phosphate buffered saline (PBS; pH 7.4), cryoprotected in 30% sucrose overnight.
At the age of 14 months, mice were anesthetized and killed by decapitation.
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