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All serum samples were stored at −80°C for up to 6 months until analysis.
Saliva samples were frozen immediately and stored at −50°C for up to 3 months until analysis.
Serum was separated from whole-blood samples by a 10 minute centrifugation at 3000 rpm and was stored afterwards at -20°C for up to two months until analysis.
About 1 g of meconium was collected from the newborns within the first 2 24 h after birth and frozen at −80°C for up to 30 months until analysis.
Patients were followed up for analysis until June 20 , 2013or until death.
Treatment duration ranged between eight and 16 months, with the majority following up until approximately 12 months.
Blood samples were stored in aliquots at −70°C until analysis (i.e. up to 21 months).
We started enrolling participants in July 2001 and continued for 57 months until March 2006, with an additional 15 months of follow-up until June 2007.
The urine samples were frozen at the hospital in SAC and kept frozen (−20°C) for up to 5 months (the 2005 samples were frozen less than 1 month) until analysis.
Those were implanted into the RVOT of 6 growing lambs followed up until 8 months.
The patient was loss to follow up until 6 months after intravitreal ranibizumab injection.
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CEO of Professional Science Editing for Scientists @ prosciediting.com