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All patients in the control group had a minimal follow-up of one year with monthly determination of serum αFP levels and imaging studies every 3 to 6 months in order to assess they did not have HCC.
In addition to the routine pregnancy controls, creatinine clearance on 24 hour urine collection is routinely required at referral; all patients undergo (at least) a monthly determination of serum creatinine and dipstick proteinuria, uric acid, urinalysis and urinary culture, serum electrolytes, coagulation and blood cell counts.
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Coral Sr/Ca time series extracted from optimal skeletal structures are highly reproducible with a mean absolute difference of 0.021 mmol mol− 1 or 0.39 °C for monthly determinations.
Patients who were diagnosed with HCC by biopsy and had monthly determinations of αFP levels were included as cases.
The exclusion criteria were: patients with incomplete files, patients who did not meet diagnosis criteria for LC or HCC, patients who were misdiagnosed, or who did not have at least three serial monthly determinations of the levels of αFP reported.
According to these, AS involves 3-monthly determinations of PSA value and digital rectal examinations (DRE) in the first 2 years after diagnosis and bi-annually thereafter [ 5].
These data were updated to year 2003 based on the monthly Preliminary Determinations of Epicenters (PDE) data available via FTP download, ftp://ghtftp.cr.usgs.gov/pub/pde/ from the USGS National Earthquake Information Center NEICC).
Follow-up continued on a monthly basis until determination of fracture union, defined in this study as painless full weight bearing and radiographic evidence of bridging callus on AP and lateral views.
These lesions were detected only because the patients were on MTX with monthly liver enzyme determinations.
Monthly observation allowed precise determination of time-dependent therapeutic response and demonstrated an unexpectedly rapid response to combination disease modifying antirheumatic drugs (DMARDs).
This approach has been used successfully in the determination of monthly gravity solutions using GRACE data, for example Rowlands et al. [ 2005]; Luthcke et al. [ 2006], and later publications.
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