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The two SSR data sets, monomorphic and polymorphic, were further analyzed to investigate a possible relationship between polymorphism level and repeat length, for each SSR type (Table 2).
Schematic of the content of monomorphic and polymorphic markers.
After the calculation of amplification sites, the number of monomorphic and polymorphic markers was depicted using a histogram (Fig. 2).
In addition, 13 loci (both monomorphic and polymorphic) possessed species-specific allele size range in A. astacus and A. leptodactylus and can be applied for detecting possible hybrids between these sister species.
Both monomorphic and polymorphic histology may be seen, and monomorphic histology appears to predict an unfavorable prognosis.
The global success rate of the assay was 66.9% (considering monomorphic and polymorphic SNPs), and a conversion rate of 51% was achieved (considering only polymorphic SNPs).
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Monomorphic, quasi-monomorphic, and polymorphic loci were defined as loci whose mean shift (bases/specimen) was equal to 0, greater than 0 but less than 1, and equal to 1 or greater, respectively, as described previously [12].
Using the different filters established in [ 20], we selected a set of markers that, in silico, were monomorphic within and polymorphic between the two sequenced genotypes and suitable for genotyping with the Illumina GoldenGate system.
However, in a mapping study of ninespine sticklebacks with the F1 individuals of a cross between parents lacking pelvic structures from phenotypically monomorphic Canadian and polymorphic Alaskan populations, the major genomic region involved in pelvic reduction was identified on LG4, which was completely distinct from the Pitx1 locus on LG7 (Shapiro et al. 2009).
Of the 19,980 SNP identified in silico [ 20], 9,043 were monomorphic within and polymorphic between the two parents of the map (Zucchini MU-CU-16 and Scallop UPV-196) and were not located in highly variable regions (filtered out with HVR4).
For conservation purposes, 28 new microsatellite loci, including 12 monomorphic and 16 polymorphic loci, were isolated from G. flabilabella.
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