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Hence, little interaction may be observed at low Aβ concentrations that consist predominantly of monomeric populations of Aβ.
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Hence, we first looked for evidences of the presence of two CtxB monomeric populations.
We found that their purified extracellular domains co-exist in stable dimeric and monomeric populations.
While dimers were virtually unbreakable, dimerization of the monomeric population was promoted through enzymatic deglycosylation.
The increase in fluorescence signal was attributed to the presence of oligomeric fluorescent species and the stronger adsorption of these larger protein oligomers to the glass surface compared to the coexisting monomeric population.
At these concentrations in aqueous solution, fluorescent Aβ (1 40) and Aβ (1 42) peptides are mainly monomeric, with small populations of dimers and trimers.
The results provide the framework for a comprehensive quantitative analysis of the effects of ALS mutations on the populations of monomeric forms of SOD1 that may be responsible for aggregation.
Thus, the long-delayed onset of familial ALS, ∼45 years on average [26], [27], might be understood, in part, by the enhanced populations of monomeric species in ALS variants (Figure 7).
Additional populations of monomeric Htt-ex1-AX647 Htt-ex1-AX647 Htt-ex1-AX647ecies were alsoligomers on the gland surface around the Htt-ex1 fibers, althouglobularlower denspeciesan at 2 h.
The enhanced populations of unfolded monomeric forms for the ALS-variants of SOD1 may provide the key.
Monomeric TERT was abundant in the populations of DNA-bound complexes from at least three of the four bulk purification samples examined here, particularly from any purification using a template-complementary oligonucleotide.
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