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Huang et al. prepared poly(4-vinylphenylboronic acid-co-pentaerythritol triacrylate) monolithic column and found that the selectivity of the monoliths increases by increasing the content of 4-vinylphenylboronic acid.
An ion pair reagent was needed because of the low affinity between the monolithic column and the anionic dyes.
On the other side, the use of monolithic column and the miniaturization of SIC work hand in hand to provide a rapid analysis.
Application is described for a system consisting of a C18-silica monolithic column and a methanol/water eluent (70/30, v/v).
They also compared sulfonate SCX hybrid monolithic columns with phosphate SCX polymer monolithic column and found a 19% increase in the phosphopeptides identification (sulfonate group bind peptide cation stronger than phosphate group) (Z. Zhang, Wang, et al. 2012a).
A continuous syngas fermentation using Clostridium carboxidivorans P7 was conducted in the MBR system under varying operational conditions, with the variables including syngas flow rate, liquid recirculation between the monolithic column and reservoir, and dilution rate.
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Many authors have compared monolithic columns and conventional packed silica columns and came to this conclusion that monolithic columns are comparable with respect to selectivity, reproducibility, and performance.
The obtained separation impedances are very small in comparison to packed bed and monolithic columns and decrease with increasing porosity.
The selection of the dimensions was made in order to correspond to slender rocking structures such as electrical equipment, ancient monolithic columns and bridges piers.
This large trans-rod eddy diffusion term is due to the combination of a large trans-rod velocity bias (≃3%), a small radial dispersion coefficient in silica monolithic columns, and a poorly designed distribution and collection of the sample streamlets at the inlet and outlet of the monolithic rod.
Future directions in modern chromatography include miniaturization of column technology with nanoparticle and monolithic columns and the use of membrane interfaces for separations.
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