A new type of supermacroporous, monolithic, cryogel affinity adsorbent was developed, allowing the specific capture of urokinase from conditioned media of human fibrosarcoma cell line HT1080.
A new type of continuous, supermacroporous, monolithic, cryogel affinity adsorbent was developed, allowing specific fractionation and separation of human peripheral blood lymphocytes in a chromatographic format.
Yang et al. prepared a macroporousboronate affinity monolithic column using a metal-organic gel as a porogenic template.
An ion pair reagent was needed because of the low affinity between the monolithic column and the anionic dyes.
AB-gel DCP based monolithic column showed remarkable adsorption affinity towards target analytes including sulfonamides (SAs) and fluorescent whitening agents (FWAs) due to its strong π-π affinity, hydrophobic effect and hydrogen bonding interaction.
Here, we present a novel approach using immuno-affinity enrichment on monolithic micro-columns in combination with state-of-the-art ultra-high pressure LC-MS/MS (UHPLC-MS/MS) detection.
Recently developed affinity chromatography on short monolithic columns seems to be a very attractive method for these purposes.
Specificity of several synthetic peptides to plasminogen activators have been studied by affinity chromatography on short monolithic columns.
Okanda and El Rassi [ 27] reported a monolithic silica column with incorporated lectins for affinity ranking in both nano-LC and capillary electrophoresis (CE).
