Suggestions(1)
Exact(2)
Monocytes were adhered to plastic 60 mm culture dishes in serum-free DMEM.
Monocytes were adhered on fibronectin-coated coverslips for 2 h and subsequently fixed with 2 % paraformaldehyde (PFA) and blocked with 3%% bovine serum albumin (BSA), 1 % HS and 10 mM glycine in PBS for 30 min at room temperature (RT).
Similar(58)
To ascertain the selective criteria that mark an irradiated EC for adhesion by monocytes, we first determined whether monocytes were adhering to irradiated ECs that were dead.
Human neutrophils and monocyte derived macrophages were adhered to plastic and pre-incubated with A2MG in the activated conformation (1 100 nM), A2MG MP (10 10) or vehicle for 15 min prior to incubation with pre-labeled E. coli (O6 K2 H1; 50:1 ratio, BacLight) 37°C, 60 min.
Ethical principles were adhered to.
Ordering etiquette must be adhered to.
* Natural justice must be adhered to.
Punctuality is adhered to.
a Freshly isolated and cryopreserved primary monocytes were allowed to adhere on TNFα stimulated or unstimulated HUVECs.
The lymphocytes were recovered, as cells in suspension, after the monocytes were left to adhere for 1 h.
Cells were transferred to tissue culture plates, and monocytes were allowed to adhere to the plastic surface for 1 hour at 37°C.
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