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Briefly, the monkeys were inoculated intravenously with approximately 105 bank vole 50% infective doses of PUUV strain Kazan-wt [19] in 1 ml of phosphate-buffered saline.
Cynomolgus monkeys were inoculated with the vaccine by subcutaneous injection 8 times with a dose of 3.2, 1.6, or 0.8 mg/kg in solution (1 ml/kg), or with the solution alone.
The monkeys were inoculated with the high-dose, TA of 3.2 mg/kg, the medium-dose, TB of 1.6 mg/kg or the low-dose, TC of 0.8 mg/kg of the vaccine.
Squirrel monkeys were inoculated intracerebrally or orally with CWD inocula (7 ).
Squirrel monkeys were inoculated with serial dilutions of the same material used to inoculate TgBo mice.
African green monkeys were inoculated with H7N9 virus and then treated intravenously with anti-human C5a antibody.
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In late April, a group of macaque monkeys was inoculated with three vaccine candidates: naked DNA, the inactivated virus from Nelson Michael's lab, and a third, "viral vector" vaccine, derived from a cold-causing virus that had been engineered in Barouch's lab to express a Zika gene.
A late seroconversion to HEV (at 6 7 weeks pi) has previously been detected in cynomolgus monkeys that were inoculated intravenously with HEV from rabbits [ 33].
Cynomolgus monkeys that were inoculated with the Dutch swine HEV showed changes that were compatible with biochemical and histological HEV-related hepatitis, including mild inflammatory reactions with multifocal lobular lymphohistiocytic infiltrates.
Six cynomolgus monkeys (Macaca fascicularis) were inoculated intravenously with swine HEV genotype 3 that was isolated from naturally and experimentally infected pigs in Brazil and the Netherlands.
To determine which species was the most sensitive for responding with disease, as measured by liver enzyme levels, eight rhesus monkeys, five cynomolgus monkeys and two chimpanzees were inoculated intravenously with the same dose (105.5 rhesus monkey ID50 of the HEV Sar-55 challenge pool) and monitored for liver enzyme elevations.
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