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In the Lancet study, by researchers from the United States and from Canada's National Microbiology Laboratory in Winnipeg, eight monkeys were injected with an extremely high dose of the virus.
The remaining monkeys were injected with the Ebola vaccine, to act as a control.
But a collaboration involving Mandeville, other Martinos Center researchers, and a lab in Belgium had recently developed a technique in which restrained monkeys were injected with an MRI contrast agent and trained to fixate quietly on a visual display.
Monkeys were injected in the femoral vein under gentle restraint.
In first instance, inactivation studies in which the monkeys were injected with a GABA-agonist (muscimol) in AIP [26] revealed a clear impairment of the grasping behavior of the hand contralateral to the inactivated hemisphere, with PG movements being the most impaired.
The monkeys were injected intramuscularly with adenoviral-based vectors that expressed codon-optimized S1 domain, M and N proteins.
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One microliter of ME20.4-saporin (0.02 μg/μL; Advanced Targeting Systems, San Diego, CA) in cholinergic lesioned monkeys or 1 μL of sterile phosphate-buffered saline (PBS) in control monkeys was injected at each site, and the needle was held in place for several seconds before moving to the next site.
Each monkey was injected with FDG immediately preceding two stressful and two secure conditions.
Fifty T75 cm flasks of C6/36 cells were infected with each of the vaccine candidates to ensure that each monkey was injected with at least 10 10 total ffu of virus/animal.
Animals were anesthetized with diazepam (5 mg/ml) and the so-called Göttinger mixture consisting of 5 ml 100 mg/ml ketamine, 1 ml 10% xylazine, 0.1 ml 1% atropine and 3.9 ml sterile water; 0.5 ml per kg body weight of the monkey was injected intramuscularly.
The performance of the optrode was then examined in the thalamus of the rat and macaque monkeys which were injected with lentiviral vectors carrying the channelrhodopsin-2-enhanced yellow fluorescent protein (ChR2-EYFP) transgene.
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monkeys were allocated
baboons were injected
monkeys were administered
monkeys were included
monkeys were boosted
monkeys was injected
monkeys were given
monkeys were rendered
monkeys were depicted
monkeys were spotted
monkeys were deprived
monkeys were rounded
monkeys were married
monkeys were discovered
monkeys were endangered
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