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Based on the recent discovery that Cdk2/Cyclin D activity is essential for the self-renewal of human ESCs and suppresses differentiation into the endoderm lineage by repressing TGF-beta-SMAD2/3 transcriptional activity (Pauklin and Vallier, 2013), this regulation likely monitors the differentiation process of human ESCs.
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In addition to stimulating and enhancing differentiation of stem cells, the excellent electrical and optical properties have also been utilized for detecting the behaviors of differentiated cells and for monitoring the differentiation process.
Based on this mechanism, Au@GO NPs monitored the differentiation in a non-destructive manner.
Furthermore, substituting CD24 for mCherry expression provides the distinct advantage of being able to further monitor the differentiation status of the NPC culture and permits tracking of terminal differentiation events.
Our transgene-free Nestin:mCherry reporter hESC line provides the unique ability to monitor the differentiation status of our cultures with the sensitivity to distinguish high and low sub-populations of Nestin expression and without requiring Nestin immunostaining.
Furthermore, Choi et al. also synthesized a scaffold assembled from GO encapsulated gold nanoparticles (Au@GO NPs) that is applicable for monitoring the differentiation of NSCs based on electrochemical detection and surface-enhanced Raman Spectroscopy (SERS) [47].
We also performed a pulse-chase experiment to monitor the differentiation of NSCs into neurons.
In order for this potential to be achieved, it is critical to efficiently monitor the differentiation of these hiPSCs into specific lineages.
In order for this potential to be achieved, it is necessary to efficiently monitor the differentiation of these iPSCs into specific lineages.
Recently, we studied the transition of HL60 promyelocytic precursor cells to the neutrophil lineage after stimulation with the inducer dimethyl sulfoxide (DMSO) by monitoring the differentiation marker CD11b (Mac-1) using flow cytometry [27].
Here, we present the quantitative use of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) as a noninvasive means to monitor the differentiation of human mesenchymal stem cells (hMSCs) using entirely endogenous sources of contrast.
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