Flow cytometric analysis was performed to monitor induction of Venus expression in T or B cell blasts over time.
To determine whether the mutated human MYC alleles behave in a manner consistent with the normal MYC gene, we monitored induction of transcription at both the murine and human MYC genes in response to serum.
A mammalian two-hybrid assay was used to monitor induction of an agonist conformation in the receptor, which allows recruitment of a peptide containing an α-helical leucine-rich motif (LxxLL) upon ligand binding [15].
To monitor induction of senescence over this time span the cells needed to be sub-cultured whenever confluence was reached.
Antioxidant defenses can be assessed by monitoring induction of manganese superoxide dismutase (MnSOD), the primary mitochondrial enzyme to scavenge matrix superoxide (Macmillan-Crow & Cruthirds, 2001).
(B ) Mouse ES cells containing a MN-specific GFP marker were used to isolate GFP expressing (GFP+) MN cells and to monitor induction of MN under different sonic hedgehog (SHH) concentrations.
Our results indicated that a programme of CNS-specific and muscle-specific gene expression accompanies the induction of neuromuscular convulsions by PTZ in 2-day-old embryos, and that this could be suppressed by co-administration of the known anticonvulsant VPA, thus providing a robust method of monitoring induction and suppression of convulsion-associated synaptic activity in the CNS.
The susceptibility of these α/β-peptide+MHC I complexes to recognition by two cognate TCRs was assessed by monitoring induction of IL-2 secretion upon exposure of T-cells to antigen-presenting cells that express H2-Kb after the latter had been incubated with the α/β-peptide.
T0: stable baseline values after monitors installation, T1: post induction of anesthesia and just before OTI, T2: end of P1, T3: end of P2, T4: end of P3, T5 and T6, respectively 1 and 5 min after the end of OTI.
To identify small molecules involved in regulation of transcription, we utilized a cell-based imaging assay that monitors the induction of a silenced GFP reporter gene.
