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The system efficiently monitors cell death dynamics, being able to detect signals related to specific death mechanisms, namely necrosis or early/late apoptosis, demonstrating a reproducible correlation between the OECT electrical response and the trends of standard cell death assays.
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At present, the development of an effective visualization tool to monitor cell death remains a significant challenge.
Characterization of cell death currently requires the use of indirect markers, which has largely limited the ability to monitor cell death processes inside the cell.
We propose and demonstrate a sensitive diagnostic device based on an Organic Electrochemical Transistor (OECT) for direct in-vitro monitoring cell death.
Time-dependent and dose-dependent dynamics of A549 cells exposed to doxorubicin are evaluated by monitoring cell death upon exposure to a range of doses and times that fully covers the protocols used in cancer treatment.
We initially expected the released luciferase in the supernatant to be the adequate source for monitoring cell death.
We monitored cell death development in infected leaves of npr1 single and atg5 npr1 double mutants.
To monitor cell death, cells were stained with propidium iodide (1.25 μg/ml) and analyzed in a FACScan (Becton Dickinson).
Chan et al. [ 9] reported that pH nanosensor-based MRI technique can monitor cell death in vivo noninvasively.
To study the fate of impaired brain tissue, we decided to monitor cell death several days after the primary injury.
To identify potential cell death modality switches upon interference with either cell death pathway, we simultaneously monitored cell death and caspase activity as a function of time.
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